Knockdown of circ_0005615 enhances the radiosensitivity of colorectal cancer by regulating the miR-665/NOTCH1 axis

Abstract Radiotherapy resistance is a challenge for colorectal cancer (CRC) treatment. Circular RNAs (circRNAs) play vital roles in the occurrence and development of CRC. This study aimed to investigate role circ_0005615 regulating radiosensitivity The levels circ_0005615, microRNA-665 (miR-665), notch receptor 1 (NOTCH1) were detected by quantitative real-time PCR or western blot. CRC cells was assessed colony formation assay. Cell viability, apoptosis, Counting Kit-8 assay, flow cytometry, migration invasion confirmed transwell assay scratch binding relationship between miR-665 NOTCH1 verified dual-luciferase reporter Xenograft used test effect on vivo . up-regulated, down-regulated tissues cells. Radiation decreased increased level. Knockdown enhanced Moreover, sponged regulate radioresistance Besides, targeted mediate Furthermore, depletion silencing elevated miR-665/NOTCH1 axis.